| 產(chǎn)品名 | 脫氧核糖核酸酶Ⅰ（牛胰） |
| 別名 | 去氧核糖核酸酶;脫氧核糖核酸; 脫氧核糖核酸酶 ;胰道酶; 胰克氧核糖核酸酶; 除氧細(xì)胞核酸酶(牛胰); 去氧細(xì)胞核酸酶 |
| 英文名 | Deoxyribonuclease |
| CAS號(hào) | 9003-98-9 |
| 分子式 | C12H22N4O6 |
| 分子量 | 318.32628 |
| EINECS編號(hào) | 232-667-0 |
| MDL號(hào) |
https://onlinelibrary.wiley.com/doi/10.1002/adfm.202315275
【穩(wěn)定性 Stability】
最佳的工作范圍是 pH 7-8，DNase 的活性依賴于 Ca2+， 并可被二價(jià)金屬離子如 Mn2+，Zn2+等激活。5mM Ca2+ 可保護(hù)酶使其不被水解，而 0.1mM Ca2+可使酶失活速 率降至原來的 1/2。
The optimal working range is pH 7-8, and the activity of DNase depends on Ca2+ and can be activated by divalent metal ions such as Mn2+, Zn2+, etc. 5mM Ca2+ can protect the enzyme from hydrolysis, while 0.1mM Ca2+ can reduce the enzyme inactivation rate to half.
【單位定義 Definition of unit】
以 1.5ml(0.15mg/ml 小牛胸腺 DNA)作底物,在 35℃且 pH5.0 的條件下，1 分鐘內(nèi)使反應(yīng)液在波長 260nm 處的 吸光度增加 0.001 所需的酶量。（一個(gè)酶活力單位以 U 表示）
Using 1.5ml (0.15mg/ml calf thymus DNA) as substrate, the required enzyme amount is required to increase the absorbance of the reaction solution by 0.001 at a wavelength of 260nm within 1 minute under the condition of 35 ℃ and pH 5.0. (One enzyme activity unit is represented by U)
【產(chǎn)品特性 Properties】
1、 Mg2+存在條件下，DNase 可隨機(jī)識(shí)別和切斷 DNA 任一條鏈上的任意位點(diǎn)；而在 Mn2+存在條件下， DNase 識(shí)別 DNA 的兩條鏈并在幾乎相同的位點(diǎn)進(jìn) 行切割；
Under the presence of Mg2+, DNase can randomly recognize and cleave any site on any strand of DNA; In the presence of Mn2+, DNase recognizes two strands of DNA and cleaves them at almost identical sites;
2、 DNase 可水解多種形式 DNA，如單鏈 DNA、雙鏈 DNA，甚至染色質(zhì)（其切割速率受組蛋白影響）。 DNase can hydrolyze various forms of DNA, such as single stranded DNA, double stranded DNA, and even chromatin (its cleavage rate is influenced by histones).
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